monolayer culture meaning in Chinese
单层培养
Examples
- Further study on the monolayer culture method of human fetal islet cells
人胎胰岛细胞单层培养方法的进一步探讨 - Cell adhesion dramatically increased after 6h by fibronectin and after 12h by collagen type i in monolayer culture
1型细胞为多角形或长校形， actin表达较少并相对聚集； 11型细胞细胞圆形，铺展良好， 。 - Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf , aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
Puc18ha和sk质粒同时经hind 、 kpn酶切后连接得中间质粒skha ；将质粒skha用bamhi酶切回收ha基因插入到插入载体pfgs11中的bamhi位点，通过酶切鉴定获得了pfgs11ha ；将含ndvf基因的质粒puc19f用hind 、 sal酶切经klenow酶补平插入到经sma酶切后的skifn中pe / l启动子下获得中间质粒skf ，再将质粒skf和puc18质粒先分别用ecor 、 xho酶切klenow补平，后再共同用sac酶切连接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位点，通过酶切鉴定获得了pe / l - f与ps - ha同向的表达载体pfgs11haf 。